It happened at 3:50 p.m. at the corner of Rohrersville Road and Appletown Road, south of Boonsboro.
Washington County Emergency Services say a box truck was involved in the crash, spilling diesel fuel onto the road.
Supervisors say the two roads were shut down for several hours for an extensive cleanup.
Maryland State Police say as of 7:24 p.m. the two victims are okay.
The scene is clear and all roads are now open.